Tools for Enterococcus faecalis research

Transposon libraries

We currently house and distribute the Enterococcus faecalis OG1RF transposon libraries that were developed in Gary Dunny's lab at the University of Minnesota.  The "SMarT library" consists of 6,829 Tn mutants spread across 73 96-well plates.  This collection is available in pooled format (ideal for TnSeq or selection assays) and in arrayed format (ideal for microtiter plate-based screens or obtaining individual mutants of interest).  The pooled library is available at no cost (other than shipping).  The full arrayed library is available for a fee to offset the labor and materials required to maintain and replicate this resource.  Individual mutants (up to 15) are available for no cost (other than shipping).

We also have an additional "SMarT" library containing ~2,000 Tn insertions in uncharacterized genes and intergenic regions.  This library is available in pooled format.

Publications

• Initial E. faecalis Tn mutagenesis work by Kristich et al:  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2423031/ 

• Arrayed library publication by Dale et al:  https://journals.asm.org/doi/10.1128/msystems.00062-18

• Replication of the arrayed Tn library by Willett et al:  https://journals.asm.org/doi/full/10.1128/spectrum.01693-23 

Antibodies

We maintain freezer stocks of the antibodies generated in the Dunny lab.  Available antibodies and other details can be found here.